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<dc:title xml:lang="en">Assembly of the giant genome of the noble crayfish and genome evolution of Decapoda</dc:title>
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<dc:subject xml:lang="en">Conservation genomics</dc:subject>
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<dcterms:abstract xml:lang="fr">Les écrevisses sont des crustacés décapodes jouant un rôle déterminant dans les écosystèmes d’eau douce mais beaucoup d’espèces européennes, en particulier l’écrevisse noble Astacus astacus, sont menacées par la peste de l’écrevisse. J’ai relevé le défi de l’assemblage du génome géant d’A. astacus (17 Gb) et obtenu un génome préliminaire de 21,8 Gb. Ce premier génome d’écrevisse européenne est l’un des plus grands génomes d’invertébrés disponibles. J’ai également exploré l’évolution des génomes de décapodes. J’ai pu montrer la contribution centrale des éléments répétés à l’expansion et la diversification des génomes de décapodes. La comparaison des protéomes a mis en évidence un core-proteome de 7 000 protéines et la diversité des histoires évolutives de leurs gènes. Mes travaux ouvrent la voie au séquençage et à la comparaison de nouveaux génomes d’écrevisses pour identifier des marqueurs de résistance à la peste et soutenir des stratégies de réintroduction et d’aquaculture durable.</dcterms:abstract>
<dcterms:abstract xml:lang="en">Crayfish are decapod crustaceans that play a decisive role in freshwater ecosystems, but many European species, particularly the noble crayfish Astacus astacus, are threatened by the crayfish plague. I took on the challenge of assembling the giant A. astacus genome (17 Gb) and obtained a preliminary genome of 21.8 Gb. This first European crayfish genome is one of the largest invertebrate genomes available. I also explored the evolution of decapod genomes. I was able to show the central contribution of repeated elements to the expansion and diversification of decapod genomes. A comparison of proteomes revealed a core-proteome of 7,000 proteins and the diversity of the evolutionary histories of their genes. My work paves the way for the sequencing and comparison of new crayfish genomes to identify markers of resistance to the plague and support strategies for reintroduction and sustainable aquaculture.</dcterms:abstract>
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